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ei dsm 15952 csm complex csm1 csm2 csm3 d32a csm4 and csm5  (Addgene inc)


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    Addgene inc ei dsm 15952 csm complex csm1 csm2 csm3 d32a csm4 and csm5
    Cryo-EM structure of the binary EiCsm complex. ( A ) Schematic diagram of the type III-A CRISPR-Cas locus of Ei. ( B ) Cryo-EM map of the binary EiCsm complex <t>(Csm1</t> 1 <t>Csm2</t> 2 <t>Csm3</t> 3 <t>Csm4</t> 1 <t>Csm5</t> 1 stoichiometry). ( C ) Schematic representation of the domain architecture of Csm1 and Csm4 subunits. ( D ) Structure of the Csm1–Csm4 subcomplex binding to the 5′ handle of the crRNA. ( E ) Close-up view of the Palm domain cOA synthetase active site in the EiCsm1 subunit.
    Ei Dsm 15952 Csm Complex Csm1 Csm2 Csm3 D32a Csm4 And Csm5, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 5 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/ei dsm 15952 csm complex csm1 csm2 csm3 d32a csm4 and csm5/product/Addgene inc
    Average 93 stars, based on 5 article reviews
    ei dsm 15952 csm complex csm1 csm2 csm3 d32a csm4 and csm5 - by Bioz Stars, 2026-03
    93/100 stars

    Images

    1) Product Images from "Mechanistic determinants and dynamics of cA6 synthesis in type III CRISPR-Cas effector complexes"

    Article Title: Mechanistic determinants and dynamics of cA6 synthesis in type III CRISPR-Cas effector complexes

    Journal: Nucleic Acids Research

    doi: 10.1093/nar/gkae1277

    Cryo-EM structure of the binary EiCsm complex. ( A ) Schematic diagram of the type III-A CRISPR-Cas locus of Ei. ( B ) Cryo-EM map of the binary EiCsm complex (Csm1 1 Csm2 2 Csm3 3 Csm4 1 Csm5 1 stoichiometry). ( C ) Schematic representation of the domain architecture of Csm1 and Csm4 subunits. ( D ) Structure of the Csm1–Csm4 subcomplex binding to the 5′ handle of the crRNA. ( E ) Close-up view of the Palm domain cOA synthetase active site in the EiCsm1 subunit.
    Figure Legend Snippet: Cryo-EM structure of the binary EiCsm complex. ( A ) Schematic diagram of the type III-A CRISPR-Cas locus of Ei. ( B ) Cryo-EM map of the binary EiCsm complex (Csm1 1 Csm2 2 Csm3 3 Csm4 1 Csm5 1 stoichiometry). ( C ) Schematic representation of the domain architecture of Csm1 and Csm4 subunits. ( D ) Structure of the Csm1–Csm4 subcomplex binding to the 5′ handle of the crRNA. ( E ) Close-up view of the Palm domain cOA synthetase active site in the EiCsm1 subunit.

    Techniques Used: Cryo-EM Sample Prep, CRISPR, Binding Assay

    Target RNA binding induces structural changes in the Csm1 Palm domain active site. ( A ) Cryo-EM map of the ternary EiCsm complex (Csm1 1 Csm2 2 Csm3 3 Csm4 1 Csm5 1 stoichiometry) bound to a CTR. ( B ) Close up view of the crRNA:CTR duplex. Flipped-out nucleobases are indicated with asterisks. ( C ) Conformational changes within EiCsm upon CTR binding, depicted as vector representation. ( D ) Structure of the Csm1–Csm4 subcomplex in the CTR-bound state. ( E ) Vector representations of the conformational changes in EiCsm1 upon CTR binding. Cartoon depicts the CTR-bound state of Csm1. ( F ) Structural overlay of the cOA synthetase active site in the binary and CTR-bound ternary EiCsm complexes.
    Figure Legend Snippet: Target RNA binding induces structural changes in the Csm1 Palm domain active site. ( A ) Cryo-EM map of the ternary EiCsm complex (Csm1 1 Csm2 2 Csm3 3 Csm4 1 Csm5 1 stoichiometry) bound to a CTR. ( B ) Close up view of the crRNA:CTR duplex. Flipped-out nucleobases are indicated with asterisks. ( C ) Conformational changes within EiCsm upon CTR binding, depicted as vector representation. ( D ) Structure of the Csm1–Csm4 subcomplex in the CTR-bound state. ( E ) Vector representations of the conformational changes in EiCsm1 upon CTR binding. Cartoon depicts the CTR-bound state of Csm1. ( F ) Structural overlay of the cOA synthetase active site in the binary and CTR-bound ternary EiCsm complexes.

    Techniques Used: RNA Binding Assay, Cryo-EM Sample Prep, Binding Assay, Plasmid Preparation



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    Cryo-EM structure of the binary EiCsm complex. ( A ) Schematic diagram of the type III-A CRISPR-Cas locus of Ei. ( B ) Cryo-EM map of the binary EiCsm complex <t>(Csm1</t> 1 <t>Csm2</t> 2 <t>Csm3</t> 3 <t>Csm4</t> 1 <t>Csm5</t> 1 stoichiometry). ( C ) Schematic representation of the domain architecture of Csm1 and Csm4 subunits. ( D ) Structure of the Csm1–Csm4 subcomplex binding to the 5′ handle of the crRNA. ( E ) Close-up view of the Palm domain cOA synthetase active site in the EiCsm1 subunit.
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    Cryo-EM structure of the binary EiCsm complex. ( A ) Schematic diagram of the type III-A CRISPR-Cas locus of Ei. ( B ) Cryo-EM map of the binary EiCsm complex <t>(Csm1</t> 1 <t>Csm2</t> 2 <t>Csm3</t> 3 <t>Csm4</t> 1 <t>Csm5</t> 1 stoichiometry). ( C ) Schematic representation of the domain architecture of Csm1 and Csm4 subunits. ( D ) Structure of the Csm1–Csm4 subcomplex binding to the 5′ handle of the crRNA. ( E ) Close-up view of the Palm domain cOA synthetase active site in the EiCsm1 subunit.
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    Cryo-EM structure of the binary EiCsm complex. ( A ) Schematic diagram of the type III-A CRISPR-Cas locus of Ei. ( B ) Cryo-EM map of the binary EiCsm complex <t>(Csm1</t> 1 <t>Csm2</t> 2 <t>Csm3</t> 3 <t>Csm4</t> 1 <t>Csm5</t> 1 stoichiometry). ( C ) Schematic representation of the domain architecture of Csm1 and Csm4 subunits. ( D ) Structure of the Csm1–Csm4 subcomplex binding to the 5′ handle of the crRNA. ( E ) Close-up view of the Palm domain cOA synthetase active site in the EiCsm1 subunit.
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    Cryo-EM structure of the binary EiCsm complex. ( A ) Schematic diagram of the type III-A CRISPR-Cas locus of Ei. ( B ) Cryo-EM map of the binary EiCsm complex <t>(Csm1</t> 1 <t>Csm2</t> 2 <t>Csm3</t> 3 <t>Csm4</t> 1 <t>Csm5</t> 1 stoichiometry). ( C ) Schematic representation of the domain architecture of Csm1 and Csm4 subunits. ( D ) Structure of the Csm1–Csm4 subcomplex binding to the 5′ handle of the crRNA. ( E ) Close-up view of the Palm domain cOA synthetase active site in the EiCsm1 subunit.
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    Image Search Results


    Cryo-EM structure of the binary EiCsm complex. ( A ) Schematic diagram of the type III-A CRISPR-Cas locus of Ei. ( B ) Cryo-EM map of the binary EiCsm complex (Csm1 1 Csm2 2 Csm3 3 Csm4 1 Csm5 1 stoichiometry). ( C ) Schematic representation of the domain architecture of Csm1 and Csm4 subunits. ( D ) Structure of the Csm1–Csm4 subcomplex binding to the 5′ handle of the crRNA. ( E ) Close-up view of the Palm domain cOA synthetase active site in the EiCsm1 subunit.

    Journal: Nucleic Acids Research

    Article Title: Mechanistic determinants and dynamics of cA6 synthesis in type III CRISPR-Cas effector complexes

    doi: 10.1093/nar/gkae1277

    Figure Lengend Snippet: Cryo-EM structure of the binary EiCsm complex. ( A ) Schematic diagram of the type III-A CRISPR-Cas locus of Ei. ( B ) Cryo-EM map of the binary EiCsm complex (Csm1 1 Csm2 2 Csm3 3 Csm4 1 Csm5 1 stoichiometry). ( C ) Schematic representation of the domain architecture of Csm1 and Csm4 subunits. ( D ) Structure of the Csm1–Csm4 subcomplex binding to the 5′ handle of the crRNA. ( E ) Close-up view of the Palm domain cOA synthetase active site in the EiCsm1 subunit.

    Article Snippet: The genes encoding for the components of the Ei (DSM-15952) Csm complex (Csm1, Csm2, Csm3 D32A , Csm4 and Csm5), together with a single spacer-containing CRISPR array, were all cloned into a single p7XNH3 vector (Addgene, #47064) as a polycistronic expression construct using FX cloning ( ).

    Techniques: Cryo-EM Sample Prep, CRISPR, Binding Assay

    Target RNA binding induces structural changes in the Csm1 Palm domain active site. ( A ) Cryo-EM map of the ternary EiCsm complex (Csm1 1 Csm2 2 Csm3 3 Csm4 1 Csm5 1 stoichiometry) bound to a CTR. ( B ) Close up view of the crRNA:CTR duplex. Flipped-out nucleobases are indicated with asterisks. ( C ) Conformational changes within EiCsm upon CTR binding, depicted as vector representation. ( D ) Structure of the Csm1–Csm4 subcomplex in the CTR-bound state. ( E ) Vector representations of the conformational changes in EiCsm1 upon CTR binding. Cartoon depicts the CTR-bound state of Csm1. ( F ) Structural overlay of the cOA synthetase active site in the binary and CTR-bound ternary EiCsm complexes.

    Journal: Nucleic Acids Research

    Article Title: Mechanistic determinants and dynamics of cA6 synthesis in type III CRISPR-Cas effector complexes

    doi: 10.1093/nar/gkae1277

    Figure Lengend Snippet: Target RNA binding induces structural changes in the Csm1 Palm domain active site. ( A ) Cryo-EM map of the ternary EiCsm complex (Csm1 1 Csm2 2 Csm3 3 Csm4 1 Csm5 1 stoichiometry) bound to a CTR. ( B ) Close up view of the crRNA:CTR duplex. Flipped-out nucleobases are indicated with asterisks. ( C ) Conformational changes within EiCsm upon CTR binding, depicted as vector representation. ( D ) Structure of the Csm1–Csm4 subcomplex in the CTR-bound state. ( E ) Vector representations of the conformational changes in EiCsm1 upon CTR binding. Cartoon depicts the CTR-bound state of Csm1. ( F ) Structural overlay of the cOA synthetase active site in the binary and CTR-bound ternary EiCsm complexes.

    Article Snippet: The genes encoding for the components of the Ei (DSM-15952) Csm complex (Csm1, Csm2, Csm3 D32A , Csm4 and Csm5), together with a single spacer-containing CRISPR array, were all cloned into a single p7XNH3 vector (Addgene, #47064) as a polycistronic expression construct using FX cloning ( ).

    Techniques: RNA Binding Assay, Cryo-EM Sample Prep, Binding Assay, Plasmid Preparation